KEY RESOURCES TABLE
| REAGENT or RESOURCE |
SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-H3K36me3 antibody | Abcam | Cat# ab9050, RRID:AB_306966 |
| Mouse monoclonal anti-H4 antibody | Abcam | Cat# ab31830, RRID:AB_1209246 |
| Rabbit polyclonal anti-SRSF11 antibody | Thermo Fisher Scientific | Cat# PA5-52606, RRID:AB_2647939 |
| Mouse anti-GAPDH | Abcam | Cat# ab9484, RRID:AB_307274 |
| Anti-mouse IgG | Cell Signaling Technology | Cat# 7076S, RRID:AB_330924 |
| Anti-rabbit IgG | Cell Signaling Technology | Cat# 7074S, RRID:AB_2099233 |
| Bacterial and Virus Strains | ||
| HSV-Set2 | This paper | N/A |
| HSV-Set2(R195G) | This paper | N/A |
| hSyn-GW-dCas9-Set2-IRES2-mCherry | This paper | N/A |
| hSyn-GW-dCas9-Set2(R195G)-IRES2-mCherry | This paper | N/A |
| P1005-GFP-Srsf11-sgRNA | This paper | N/A |
| P1005-GFP-NT-sgRNA | This paper | N/A |
| Deposited Data | ||
| RNA-sequencing of mouse NAc following 21 days of cocaine self-administration | This paper | GSE141520 |
| RNA-sequencing of mouse NAc following dCas9-Set2 + Srsf11-sgRNA | This paper | GSE180988 |
| RNA-sequencing of mouse NAc following dCas9-Set2 + Srsf11-sgRNA | This paper | GSE180988 |
| RNA-sequencing of mouse NAc following 21 days of saline self-administration | This paper | GSE141520 |
| Cut&Run sequencing of H3K36me3 of dCas9-SET2 transfected N2a cells | This paper | GSE180988 |
| ChIP-sequencing of H3K36me3 of mouse NAc following cocaine SA | This paper | GSE180988 |
| ChIP-sequencing of H3K36me3 of mouse NAc following HSV-Set2 transfection | This paper | GSE180988 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664) |
| Oligonucleotides | ||
| PCR Primers, see Table S1 | This paper | N/A |
| sgRNA, see Table S1 | This paper | N/A |
| Software and Algorithms | ||
| Prism v8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| R v4.0.2 | The R Project for Statistical Computing | https://www.r-project.org/ |
| Factor Analysis - Cocaine Self Administration | Walker et al., 2018 | N/A |
| CUT&RUN Analysis | Skene and Henikoff, 2017 | N/A |
| Splicing analysis rMATS | Shen et al., 2014 | http://rnaseq-mats.sourceforge.net/ |
| Splicing analysis MAJIQ | Norton et al., 2017 | https://majiq.biociphers.org/ |
| DiffBind | Ross-Innes et al., 2012 | Bioconductor https://bioconductor.org/packages/release/bioc/html/DiffBind.html |
| Deseq2 | Love et al., 2014 | Bioconductor https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| SICER | Xu et al., 2014b | https://zanglab.github.io/SICER2/ |
| HOMER | Heinz et al., 2010 | http://homer.ucsd.edu/homer/motif/ |