Table 1.
List of antibodies used for immunofluorescent staining
| Antibody | Source | Identifier | Dilution | Use |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti-Brn-3a | Santa Cruz | Cat# sc-31984 | 1:250 | Whole mounts |
| Anti-GFAP | Chemicon | Cat# MAB360 | 1:500 | Immunocytochemistry |
| Anti-Iba1 | Wako | Cat# 019-19741 | 1:500 | Whole mounts, cryosections, immunocytochemistry |
| Anti-Iba1 | Abcam | Cat# AB48004 | 1:200 | Immunocytochemistry |
| Anti-P2X7 Receptor | Alomone | Cat# APR-008 | 1:200 | Immunocytochemistry |
| Anti-Synaptophysin | Thermo Fisher | Cat# MA5-14532 | 1:250 | Immunocytochemistry |
| Secondary antibodies | ||||
| Anti-Mouse Alexa Fluor 488 | Thermo Fisher | Cat# A21202 | 1:500 | Whole mounts |
| Anti-Rabbit Alexa Fluor 488 | Thermo Fisher | Cat# A21206 | 1:500 | Immunocytochemistry |
| Anti-Mouse Alexa Fluor 488 | Thermo Fisher | Cat# A11001 | 1:500 | Immunocytochemistry |
| Anti-Goat Alexa Fluor 488 | Thermo Fisher | Cat# A11055 | 1:500 | Immunocytochemistry |
| Anti-Rabbit Alexa Fluor 546 | Thermo Fisher | Cat# A11035 | 1:500 | Immunocytochemistry |
| Anti-Goat Alexa Fluor 555 | Thermo Fisher | Cat# A21432 | 1:500 | Immunocytochemistry |
| Anti-Rabbit Alexa Fluor 555 | Thermo Fisher | Cat# A31572 | 1:500 | Whole mounts, immunocytochemistry |
| Anti-Goat Alexa Fluor 555 | Thermo Fisher | Cat# A10042 | 1:500 | Cryosections |