Figure 2.

Effect of phthalate mixture exposure on luteinization. Early antral follicles from adult CD-1 mice were cultured in FSH supplemented media and treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 1–500µg/ml) for 96 h prior to hCG treatment. Follicles were then treated with or without hCG maturation media and with or without PHTmix. Follicles were collected at 18 h post-hCG treatment and were subjected to qPCR to measure the mRNA levels of Cdkn1a (A) and Wnt4 (B). Data are presented as a percent fold change relative to the hCG alone control group. Graphs represent mean ± SEM from 3–9 experiments, with 10–12 follicles/treatment group in each experiment. Bars that do not share a letter designation are significantly different (p ≤ .05).