Figure 3.
Effect of phthalate mixture exposure on prostaglandin (PG) levels. Early antral follicles from adult CD-1 mice were cultured in FSH supplemented media and treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 1–500µg/ml) for 96 h prior to hCG treatment. Follicles were then treated with or without hCG maturation media and with or without PHTmix. Media were collected at multiple time-points (4 and 11 h) post-hCG treatment, and PGE2 (A) and PGF2α (B) levels were measured via an ELISA. Data are presented as a percent change relative to the hCG alone control group. Graphs represent mean ± SEM from 3 to 10 experiments, with 10 to 12 follicles/treatment group in each experiment. Bars that do not share a letter designation are significantly different (p ≤ .05).