Figure 4.

Effect of phthalate mixture exposure on prostaglandin synthesis, metabolism, and transport. Early antral follicles from adult CD-1 mice were cultured in FSH supplemented media and treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 1–500µg/ml) for 96 hr prior to hCG treatment. Follicles were then treated with or without hCG maturation media and with or without PHTmix. Follicles were collected at multiple time-points (4 and 11 h) post-hCG treatment and were subjected to qPCR to measure the mRNA levels of Pla2g4a (A), Ptgs2 (B), Hpgd (C), and Slco2a1 (D). Data are presented as a percent fold change relative to the hCG alone control group. Graphs represent mean ± SEM from 3–10 experiments, with 10–12 follicles/treatment group in each experiment. Bars that do not share a letter designation are significantly different (p ≤ .05).