Table 1.
Primer Information
| SYBR Primer Sequences | |||
|---|---|---|---|
| Gene Symbol | Gene Name | F/R | Primer Sequence |
| Abcc4 | ATP-binding cassette, sub-family C (CFTR/MRP), member 4 | F | 5′-TTCTGGTTATTCTTCTGCCTCTG-3′ |
| R | 5′-CCCACGCATACATCTTTATTATCC-3′ | ||
| Actb | Actin, beta | F | 5′-GGGCACAGTGTGGGTGAC-3′ |
| R | 5′-CTGGCACCACACCTTCTAC-3′ | ||
| Adamts1 | A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 | F | 5′-CAGTACCAGACCTTGTGCAGACCTT-3′ |
| R | 5′-CACACCTCACTGCTTACTGGTTTGA-3′ | ||
| Cdkn1a | Cyclin-dependent kinase inhibitor 1 A (P21) | F | 5′-TTAGGCAGGCTCCAGTGGCAACC-3′ |
| R | 5′-ACCCCCACCACCACACACCATA-3′ | ||
| Cyp11a1 | Cytochrome P450, family 11, subfamily a, polypeptide 1 | F | 5′-AGATCCCTTCCCCTGGTGACAATG-3′ |
| R | 5′-CGCATGAGAGTATCGACGCATC-3′ | ||
| Edn2 | Endothelin 2 | F | 5′-CTCCTGGCTTGACAAGGAATG-3′ |
| R | 5′-GCTGTCTGTCCCGCAGTGTT-3′ | ||
| Hpgd | Hydroxyprostaglandin dehydrogenase 15 (NAD) | F | 5′-CACCTCCGTTTTGCTTACTCA-3′ |
| R | 5′-GTTCGTCCAGTGTGATGTGG-3′ | ||
| Hsd3b1 | Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 | F | 5′-CAGGAGAAAGAACTGCAGGAGGTC-3′ |
| R | 5′-GCACACTTGCTTGAACACAGGC-3′ | ||
| Il6 | interleukin 6 | F | 5′-GATGCTACCAAACTGGATATAATC-3′ |
| R | 5′-GGTCCTTAGCCACTCCTTCTGTG -3′ | ||
| Mmp9 | Matrix metallopeptidase 9 | F | 5′-GATCCCCAGAGCGTCATTC-3′ |
| R | 5′-CCACCTTGTTCACCTCATTTTG-3′ | ||
| Mmp14 | Matrix metallopeptidase 14 (membrane-inserted) | F | 5′-CAGTATGGCTACCTACCTCCAG-3′ |
| R | 5′-GCCTTGCCTGTCACTTGTAAA-3′ | ||
| Mmp16 | Matrix metallopeptidase 16 | F | 5′-TTACTCGCATTCAGCTCTGGA-3′ |
| R | 5′-CCGCAGACTGTAGCACATAAAA-3′ | ||
| Mmp19 | Matrix metallopeptidase 19 | F | 5′- GACAGCAAAGACCTGGAGGATTA -3′ |
| R | 5′- CTGACCGGAAATGGGCAGT -3′ | ||
| Parm1 | Prostate androgen-regulated mucin-like protein 1 | F | 5′-ACCTGAAGATCAGGCACTCC-3′ |
| R | 5′-CCTCAGCCACCTTTCTTCGT-3′ | ||
| Pla2g4a | Phospholipase A2, group IVA (cytosolic, calcium-dependent) | F | 5′-CAGCAGGAAGCGAACGAGAC-3′ |
| R | 5′-GACGTAGTTGGCATCCATCAGT-3′ | ||
| Plat | Plasminogen activator, tissue | F | 5‘- AGGAGGACTCTACACAGACATCACCTC -3′ |
| R | 5′-ATCGTCATCAAATTCCTCATGGACTATG-3′ | ||
| Plau | Plasminogen activator, urokinase | F | 5′-GTTCAGACTGTGAGATCACTGG -3′ |
| R | 5′-CAGAGAGGACGGTCAGCATGG-3′ | ||
| Ptges | Prostaglandin E synthase | F | 5′-ATCAAGATGTACGCGGTGGCT-3′ |
| R | 5′-GATTGTCTCCATGTCGTTGCG-3′ | ||
| Serpinb2 | Serine (or cysteine) peptidase inhibitor, clade B, member 2 | F | 5′-CCGCTCAGAAGATAACGAGATTG-3′ |
| R | 5′-TGGCCAATGTTGATGAGATGC-3′ | ||
| Serpine1 | Serine (or cysteine) peptidase inhibitor, clade E, member 1 | F | 5′-CCCACACAGCCCATCAGG-3′ |
| R | 5′-CCGAGGACACGCCATAGG-3′ | ||
| Slco2a1 | Solute carrier organic anion transporter family, member 2a1 | F | 5′-TCGCCTCTGTATATCTCCATC-3′ |
| R | 5′-GTAGCCGTGTCCACTCTG-3′ | ||
| Star | Steroidogenic acute regulatory protein | F | 5′-CAGGGAGAGGTGGCTATGCA-3′ |
| R | 5′-CCGTGTCTTTTCCAATCCTCTG-3′ | ||
| Timp1 | Tissue inhibitor of metalloproteinase 1 | F | 5′-CGAGACCACCTTATACCAGCG-3′ |
| R | 5′-ATGACTGGGGTGTAGGCGTA-3′ | ||
| Wnt4 | Wingless-type MMTV integration site family, member 4 | F | 5′-CAGGAAGGCCATCTTGACACACA-3′ |
| R | 5′-TGGCACCGTCAAACTTCTCC-3′ | ||
| TaqMan Primer Information | |||
| Gene Symbol | Gene Name | Assay ID | |
| Pgr | Progesterone receptor | Mm00435628_m1 | |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | Mm00478374_m1 | |