Fig. 5.

Images of islet viability after thawing. Non-encapsulated, 1.75%, and 2.5% encapsulated islets were cryopreserved and thawed after 4 weeks. Viability staining was done with Calcein AM (green for viable cells), propidium iodide (red for dead cells), and 4′,6-diamidino-2-phenylindole (blue as nuclear counterstain). A representative image was taken using a confocal microscope after reading the fluorescence intensity on a fluorescent plate reader.