Table 1.

In Vitro Characterization of Pre-freezing, Post-thawing, and Post-recovery Culture of Cryopreserved Islets.

		Yield	Viability (IE)	Stimulation index
Free islets	Pre freeze	100%	92 ± 4.1%	3.5 ± 0.2
	Post thaw	72.6 ± 5.7%	78 ± 2.4%
	Post 48 h culture	48 ± 5.7%	52 ± 7.4%	1.9 ± 0.5
1.75% alginate	Post thaw	84.6 ± 4.1%	88 ± 2.0%
	Post 48 h culture	79 ± 1.7%	83 ± 3.7%	2.9 ± 0.1
2.5% alginate	Post thaw	83.6 ± 3.3%	85 ± 2.9%
	Post 48 h culture	80.4 ± 2.6%	80 ± 1.7%	3.3 ± 0.3

Islet yield was calculated by counting dithizone staining and expressed as percentage recovery from the initial number prior to freezing. Viability was shown as a count of viable islets using fluorescein diacetate and propidium iodide staining. Stimulation index was calculated as the ratio of insulin produced after high glucose stimulation compared to low glucose stimulation of the islets. Each result represents the average value for 5 samples.