Figure 2.

Liver and intestine respond to systemic increase in neutrophil extracellular trap (NET) levels and secrete DNase1 and DNase1L3, respectively. A, qPCR-based analysis of gene expression of DNase1 and DNase1L3 in indicated organs of mice injected either vehicle or NET. n=5 mice per group. B, Single radial enzyme diffusion (SRED) assay to measure total DNase activity in liver and intestine of vehicle or NET injected mice. C, Denaturing polyacrylamide gel electrophoresis zymography (DPZ) for quantification of DNase1 levels in lysates of liver (top) and intestine (bottom) of mice injected with either vehicle or NET. D and E, HepG2 cells (Hepatocytes) or Caco2 cells (Enterocytes) were incubated without or with NET and the cell culture supernatants were assayed for total DNase activity by SRED (D) or DNase1 and DNase1L3 activity by DPZ (E). The data from 3 independent biological replicates are presented. Data in (A) and (B) were analyzed for normality using Shapiro-Wilk test and P were calculated using Mann-Whitney U test (A) or Student 2-tailed t test (B).