Figure 4.

Interactional Network of Antioxidant Defense in Seahorse Brood Pouch Development

(A) Boxplots showing the changing tendency of one oxidant and two antioxidants over different pouch developmental stages in seahorses. Each point represents one replicate (n = 12). Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× the interquartile range.

(B and C) Significant correlations between significantly different antioxidants and DEGs (correlation coefficient >0.9 and p < 0.01). Fonts of antioxidants and genes involved in transporting or related to RA are in bold. Metabolites and genes are indicated with blue and green background colors, respectively. Blue and green lines indicate significant positive and negative correlations, respectively. (B) Correlations between canthaxanthin and ophthalmic acid with other DEGs identified for brood pouch formation. (C) Correlations between canthaxanthin (left) and astaxanthin (right) with other DEGs identified during pregnancy.