In vivo analysis of the inflammatory status
(A) SEM images of macrophages on different samples after implantation for 7 days.
(B) Immunofluorescent staining images of macrophages on different samples after implantation for 7 days. Red, green, and blue fluorescence reflect density for iNOS, CD163, and nuclei.
(C) H&E staining images of peri-implant tissues after implantation for 7 days. The fibrous layers are marked by dashed lines.
(D) Quantitative comparison of fibrous layer thickness after implantation for 1, 3, and 7 days. Scale bars, 100 μm (except those in the insets being equal to 10 μm). ∗∗p < 0.01 and ∗∗∗p < 0.001 compared with the P group, whereas ##p < 0.01 and ###p < 0.001 compared with the P-DPI group (n = 6).