In vivo analysis of bone regeneration after implantation for 8 weeks
(A) 2D and reconstructed 3D micro-computed tomography (micro-CT) images of the bone with the implants inside.
(B–D) Quantitative micro-CT data of (B) bone volume/total volume (BV/TV), (C) trabecular number (Tb.N), and (D) trabecular separation (Tb.Sp).
(E) Formation of new bone labeled by sequential fluorescent staining (tissues labeled by alizarin red, tetracycline hydrochloride, and calcein show red, yellow, and green fluorescence, respectively).
(F and G) Histological observation of peri-implant tissues after (F) Van Gieson and (G) H&E staining with new bone marked by dashed lines. Scale bars, 100 μm. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with the P group, whereas ##p < 0.01 and ###p < 0.001 compared with the P-DPI group (n = 6).