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Synthesis of rcDNA and dslDNA.
As the template, pgRNA is preformed for protein priming (A) mediated by the TP domain of Pol, and the (−)-DNA is subsequently synthesized (B). For the synthesis of rcDNA (C), the RNA primer is transferred to DR2 of (−)-DNA and primes the synthesis of (+)-DNA. For the synthesis of dslDNA (D), the RNA primer performs the direct (in situ) extension yielding (+)-DNA. See text for detailed description.
