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p160 coactivator function with full-length AR does not correlate with binding to AR AF-2. CV-1 cells were transiently transfected with 0.25 μg of pSVAR0, 0.5 μg of MMTV-LUC reporter gene, and 1.25 μg of pSG5.HA (no CoA) or the indicated pSG5.HA-coactivator expression vector. Luciferase activity was measured after cells were grown with or without 20 nM DHT.
