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NR box-independent and AD1-independent enhancement of AR AF-1 activity by GRIP1 and its C-terminal fragment. CV-1 cells were cotransfected with 0.25 μg of pM vector encoding Gal4DBD-ARAF1, 0.5 μg of GK1 reporter gene, and the indicated amount of pSG5.HA vector encoding GRIP1 or GRIP1 fragments. Luciferase activity of the cell extracts is shown.
