FIGURE 3.

Presence of the UEA lineage in Chondrichthyan species via Southern blotting. BamHI-digested DNA from different species was hybridized with the nurse shark MHC class I UEA α3 domain probe under low-stringency conditions. The chondrichthyan species used were one chimaera (spotted ratfish H. colliei), four rays (cownose ray R. bonasus, little skate L. erinacea, thornback ray R. clavata, and marbled electric ray T. marmorata), and eight sharks (nurse shark G. cirratum, bamboo shark C. punctatum, horn shark H.s francisci, sand-tiger shark C. taurus, lesser spotted dogfish S. canicula, lemon shark N. brevirostris, spiny dogfish S. acanthias, and blue shark P. glauca). The marker size (kb) is shown on the left.