Figure 10.

Differentially expressed genes in P15 brainstem tissue following repeated neonatal morphine exposure. A–C, The effect of morphine exposure on gene expression relative to saline controls in the sex-collapsed dataset [A; n = 6 saline (3 females, 3 males), n = 6 morphine (3 females, 3 males)], males only (B; n = 3 saline, n = 3 morphine), and females only (C; n = 3 saline, n = 3 morphine). The x-axis shows gene expression as LogFC, and the y-axis shows Log₁₀ p values (unadjusted). Color coding of individual genes represents LogFC > 0.26 (green), p < 0.05 (blue), LogFC > 0.26 and p < 0.05 (red), and nonsignificant (NS; gray). Complete gene lists and expression data from RNA-seq analysis are presented in Extended Data Figure 10-1 (both sexes; all genes), Extended Data Figure 10-2 (both sexes; LogFC > 0.26, p value < 0.05), Extended Data Figure 10-3 (females; all genes), Extended Data Figure 10-4 (females LogFC > 0.26, p value < 0.05); Extended Data Figure 10-5 (males; all genes), Extended Data Figure 10-6 (males; LogFC > 0.26, p-value < 0.05). Data from qPCR validation of differentially expressed genes (from the sex-collapsed analysis) are presented in Extended Data Figure 10-7.