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. 2021 Sep 21;16(9):e0257537. doi: 10.1371/journal.pone.0257537

Fig 6. Pooled screening for B2AR.

Fig 6

A. Design of the experiment. The sgRNA library containing 128 sgRNAs was infected into clone HEK293T + dCas9-AID*Δ + 6xCRE mCherry reporter at about 0.05 MOI. Cells were selected with puromycin, and treated with isoproterenol to stimulate B2AR activity. Cell pellets were obtained for genomic extraction before sorting (presort cells) and sorted for the mCherry negative in both populations (the infected with the B2AR library and the non-infected cells). WT HEK293T cells do not contain the CRISPR-X system, and are used here as negative control. B. mCherry fluorescence intensity histogram, showing the cut-off for sorting mCherry negative cells.