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. 2021 Sep 21;16(9):e0257537. doi: 10.1371/journal.pone.0257537

Fig 8. The C184Y mutation reduces B2AR sensitivity to isoproterenol.

Fig 8

Reporter activity to titrating concentrations of isoproterenol of cells with or without endogenous B2AR (HEK-CRE-mCherry or HEK-CRE-mCherry B2AR-KO, respectively) after transient transfection of B2AR-wt or B2AR-C184Y. CRE-mCherry activity was assayed 12 hours after isoproterenol stimulation (other time points are shown in S5 Fig). Isoproterenol dependent CRE-mCherry activity is expressed as a percentage of 10μM forskolin (used as internal positive control, as it activates cAMP downstream of B2AR) response. Results are plotted of mean data of N = 3 ± SEM.