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. Author manuscript; available in PMC: 2021 Sep 21.
Published in final edited form as: Sci Signal. 2020 Jul 7;13(639):eaax6313. doi: 10.1126/scisignal.aax6313

Fig. 5. P-loop cysteine of AtFN3K (Cys32) is critical for the formation of disulfide-linked dimer species.

Fig. 5.

(A) Non-reducing SDS-PAGE of WT and Cys-to-Ala mutant AtFN3K. 15 μg of protein was incubated with 1 mM DTT or 1mM H2O2 for 20 min and then subjected to SDS-PAGE under non-reducing conditions. DS-S: Disulfide linked dimer; MRed: Monomer Reduced; MS-S: Monomer with intramolecular disulfide. Blots are representative of 3 experiments. (B) Multiple sequence alignment of FN3K orthologs. Two additional cysteines (Cys196 and Cys222) specific to plant FN3Ks are shown. The alignment was generated using MUSCLE (68).