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. Author manuscript; available in PMC: 2021 Sep 21.
Published in final edited form as: Sci Signal. 2020 Jul 7;13(639):eaax6313. doi: 10.1126/scisignal.aax6313

Fig. 7. P-loop cysteine contributes to redox-sensitivity in HsFN3K.

Fig. 7.

(A and B) PK/LDH assays performed with 10.0 μg of HsFN3K (A) or 1.0 μg each of TfFN3K and L. plantarum FN3K (LpFN3K) (B). Proteins were incubated with buffer (0 mM DTT) or 2 mM DTT, and ribulose-N-α-Ac-lysine was used as the substrate. Data are mean ± standard error of three independent experiments. (C) Effect of different diamide concentrations on transfected Flag-tagged WT and C24A HsFN3K in HEK293 cells. Total cell lysates were immunoblotted for Flag. DS-S: disulfide-linked dimer, M: monomer. Blot is representative of 3 experiments.