KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse polyclonal anti-Rad51 (IF and WB) | Abcam | Cat# ab88572; RRID: AB_2042762 |
| Mouse monoclonal anti-BRCA1 (IF and WB) | Santa Cruz Bio | Cat# sc-6954; RRID: AB_626761 |
| Mouse monoclonal anti-phospho-Histone H2A.X (Ser139), clone JBW301, (IF and WB) | Millipore | Cat# 05-636; RRID: AB_309864 |
| Rabbit monoclonal anti-HA-Tag (C29F4) (IF) | Cell Signaling Technology | Cat# 3724; RRID: AB_1549585 |
| Mouse monoclonal anti-HA.11 Epitope Tag Antibody (WB) | Biolegend | Cat# 901515; RRID: AB_2565334 |
| Mouse monoclonal anti-Flag, Clone M2, (IF) | Sigma-Aldrich | Cat# F1804; RRID: AB_262044 |
| Rabbit polyclonal anti-Flag Tag (WB) | Cell Signaling Technology | Cat# 2368; RRID: AB_2217020 |
| Mouse monoclonal anti-Myc-Tag (9B11) (IF, WB) | Cell Signaling Technology | Cat# 2276; RRID: AB_331783 |
| Mouse monoclonal anti-LOC340602(CXorf67) (D-1) (IF, WB and IHC) | Santa Cruz Bio | Cat# sc-515296 |
| Normal mouse IgG | Santa Cruz Bio | Cat# sc-2025; RRID: AB_737182 |
| Mouse mixture of two monoclonal anti-Green Fluorescent Protein (7.1 and 13.1) (WB) | Roche | Cat# 11814460001; RRID: AB_390913 |
| Rabbit monoclonal anti-histone H3 (D2B12) (WB) | Cell Signaling Technology | Cat# 4620; RRID: AB_1904005 |
| Rabbit polyclonal anti-PALB2 (WB) | Abcam | Cat# ab220861 |
| Mouse monoclonal anti-BRCA2 (Clone 234403) (WB) | R&D Systems | Cat# MAB2476; RRID: AB_2259370 |
| Rabbit polyclonal anti-KU80 (WB) | Cell Signaling Technology | Cat# 2753; RRID: AB_2257526 |
| Rabbit polyclonal anti-H2A.X (WB) | Abcam | Cat# 11175; RRID: AB_297814 |
| Mouse monoclonal anti-β-Actin (WB) | Santa Cruz Bio | Cat# sc-47778; RRID: AB_626632 |
| Mouse monoclonal anti-GST (WB) | Sigma-Aldrich | Cat# G1166; RRID: AB_259846 |
| Mouse monoclonal anti-His (WB) | Sigma-Aldrich | Cat# H1029; RRID: AB_260015 |
| Rabbit polyclonal anti-GAPDH | Bioworld Technology | Cat# AP0063; RRID: AB_2651132 |
| Rabbit polyclonal anti-RPA2 | ABclonal | Cat# A2189; RRID: AB_2764207 |
| Mouse monoclonal anti-β-Tubilin | Sigma-Aldrich | Cat# T5293; RRID: AB_477580 |
| Bacterial and Virus Strains | ||
| DH10Bac chemically compotent cell | Shanghai Weidi Biotechnology | Cat# DL1071 |
| Escherichia coli DH5α | Tiangen Biotech | Cat# CB101 |
| Escherichia coli BL21 (DE3) | Tiangen Biotech | Cat# CB105 |
| Biological Samples | ||
| Ependymoma tumor samples | Children’s Hospital of Fudan University | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| D-Luciferin | Perkin Elmer | Cat# 122799 |
| Niraparib | MedChem Express | Cat# HY-10619 |
| CXorf67 (420-432aa) peptide | This paper | N/A |
| CXorf67 (420-432aa) W425C peptide | This paper | N/A |
| Olaparib | MedChem Express | Cat# HY-10162 |
| DAPI | Sigma-Aldrich | Cat# D8417 |
| EGF | Gibco | Cat# PHG0311 |
| FGF | Gibco | Cat# PHG0261 |
| DMAC | Sangon Biotech | Cat# A504006 |
| Solutol HS-15 | MedChem Express | Cat# HY-Y1893 |
| SBE-β-CD | MedChem Express | Cat# HY-17031 |
| Methylcellulose | MedChem Express | Cat# HY-125861 |
| Talazoparib | MedChem Express | Cat# HY-16106 |
| Critical Commercial Assays | ||
| CellTiter-Glo Kit | Promega | Cat# G7572 |
| H&E staining Kit | Beyotime | Cat# C0105 |
| ClonExpress II one step cloning Kit | Vazyme | Cat# C112 |
| Lipofectamine 3000 transfection Kit | Invitrogen | Cat# L3000015 |
| DAB Kit | Beyotime | Cat# P0203 |
| FITC Annexin V Apoptosis Detection Kit | BD Biosciences | Cat# 556547 |
| OxiSelect Comet Assay Kit | Cell Biolabs | Cat# STA-350 |
| Deposited Data | ||
| Microarray gene expression | (Donson et al., 2017; Pajtler et al., 2015) | GEO: GSE64415 and GSE94349 |
| TCGA mRNA data and clinical data | TCGA | https://portal.gdc.cancer.gov |
| The raw data of this study | This paper | https://doi.org/10.17632/rjxfxzsh5m.1 |
| CellMinerCDB | (Rajapakse et al., 2018) | https://discover.nci.nih.gov/cellminercdb/ |
| Experimental Models: Cell Lines | ||
| Human: U2OS DR-GFP | (Lou etal., 2017) | N/A |
| Human: U2OS-LacO | (Zhou etal., 2017) | N/A |
| Human: U2OS | ATCC | Cat# HTB-96; RRID: CVCL_0042 |
| Human: Daoy | Cell library, SIBCB | SCSP-509 |
| Human: 293T | Cell library, SIBCB | SCSP-502 |
| Ependymoma primary cell cultures: PFA-1 and PFA-2 | This paper | N/A |
| Ependymoma cell cultures from xenograft: PFA-3 and PFA-4 | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.CB17-Prkdcscid/NcrCrl (NOD-SCID) | Vital River Laboratories | Cat# 406 |
| Mouse: BALB/cASlac-nu | SLAC Laboratory Animal | N/A |
| Oligonucleotides | ||
| siRNA targeting sequence: BRCA2: GAAGAACAAUAUCCUACUA | This paper | N/A |
| siRNA targeting sequence: Ku80: GCGAGUAACCAGCUCAUAA | This paper | N/A |
| Non-targeting control siRNA sequence: UUCUCCGAACGUGUCACGUUU | This paper | N/A |
| siRNA targeting sequence: CXorf67 #1: GGAGGGCUGAACAACGAAAUU | This paper | N/A |
| siRNA targeting sequence: CXorf67 #2: GCUGCUGUGCUGACGGAGAUU | This paper | N/A |
| Recombinant DNA | ||
| GFP/Myc-LacR | (Zhou etal., 2017) | N/A |
| pEGFP-CXorf67 | This paper | N/A |
| pCMV-CXorf67-HA | This paper | N/A |
| pCMV-CXorf67-Flag | This paper | N/A |
| Lentiviral pLVX-CXorf67 | This paper | N/A |
| Lentiviral pLKO.1-puro | Addgene | Cat# 8453; RRID: Addgene_8453 |
| pSpCas9(BB)-2A-GFP (PX458) | Addgene | Cat# 48138; RRID: Addgene_48138 |
| pCBASceI | Addgene | Cat# 26477; RRID: Addgene_26477 |
| Software and Algorithms | ||
| FlowJo (v10.4) | FlowJo software | https://www.flowjo.com |
| Leica LAS AF software (LAS AF 2.4.1) | Leica | In house license |
| NIS-Elements software (v4.5) | Nikon | In house license |
| GraphPad Prism (v6.0) | GraphPad software lnc. | https://www.graphpad.com |
| ImageJ software (v1.8.0) | National Institutes of Health | https://imagej.nih.gov/ij/ |
| CometScore software (v2.0) | Rex Hoover | http://rexhoover.com/index.php?id=cometscore |
| Living Image Software (v4.5.4) | Perkin Elmer | In house license |