Fig. 4.

Polyamide 1 suppresses the ERRα-dependent luciferase expression. Mouse hepatocytes stably expression cytochrome c-driven luciferase were treated with polyamide 1 or 2 for 24 h at indicated dose. The cells were then lysed and used to determine the luciferase activity using a luminometer. Polyamide 1 treatment at 1 μM reduced the luciferase activity whereas polyamide 2 did not. Neg con, the mouse hepatocyte without luciferase expression vector; Naive, untreated cells expressing the luciferase reporter; Veh con, cells expressing the luciferase reporter treated with vehicle. n = 3. ** statistically significant at p < 0.01 when compared to vehicle control group