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. Author manuscript; available in PMC: 2021 Sep 21.
Published in final edited form as: Med Chem Res. 2020 Feb 26;29(4):607–616. doi: 10.1007/s00044-019-02493-4

Fig. 7.

Fig. 7

Specificity for Polyamide 1 at reducing mitochondrial respiration. a The effect of polyamide 1 (1 μM) was compared with that of the mismatch polyamide 2 (1 μM) and an ERRα inverse agonist, XCT-790 (2 μM). Con, vehicle-treated cells. Arrows indicated time points when these chemicals were added. n = 3. Top, real-time plots for OCR levels, each data point was replication of four readings for each sample with three samples each group for each experiment. Bottom left, the basal respiration was calculated by averaging the baseline OCR minus the nonmitochondrial OCR; bottom right, the maximal respiratory capacity was calculated by averaging the maximal OCR minus the nonmitochondrial OCR. b Comparison of ERRα levels in Huh7 cells treated with polyamide 1 vs. polyamide 2 and controls. **p < 0.01 difference between experimental and control groups. c Comparison of cytochrome c (CytC) gene expression in Huh7 cells treated with polyamide 1 vs. polyamide 2 and controls