Figure 2. Broad protection against infection of clinical strains by intranasal immunization of inactivated whole cell (IWC) of A. baumannii.

(A) Mice were immunized intranasally (i.n.) with IWC of LAC-4 on day 0. On day 7, mice were infected intratracheally (i.t.) with lethal dose of clinical strains of A. baumannii SJZ24 or SJZ26 (n = 10). (B) Mice were immunized i.n. with IWC of SJZ24, SJZ26, SJZ09, or ATCC17978 and challenged with lethal dose of LAC-4 7 days later. Survival rate of mice was monitored for 7 days after challenge. n = 10. **p<0.01, ****p<0.0001 determined by log-rank test. These experiments were repeated at least twice.