Skip to main content
. 2021 Sep 20;10:e69951. doi: 10.7554/eLife.69951

Figure 3. Rapid immune memory induced by a single intranasal vaccination.

Figure 3.

(A) Dynamic responses of TNF-α and IL-6 in the lungs of A. baumannii inactivated whole cell (IWC) (LAC-4)-immunized mice (n = 3 per timepoint). (B–D) IWC (LAC-4)-immunized mice were challenged intratracheally (i.t.) with LAC-4 at day 7 after immunization. (B) Levels of TNF-α, IL-6, and IL-1β at 0 hr and 2 hr post infection (hpi) in the lungs were measured by ELISA. (C) Transcriptional levels of chemokines in the lungs at 0 hr and 2 hpi were assessed by real-time PCR. (D) Numbers of neutrophils, monocytes, and macrophages in the lungs of mice were determined by flow cytometry. Data are presented as mean ± SD. (n = 3–4 mice/group). *p<0.05; **p<0.01, ***p<0.001, ****p<0.0001, ordinary two-way ANOVA. Data are representative of two independent experiments.

Figure 3—source data 1. Raw data for Figure 3.