| Genetic reagent (Mus musculus) |
C57BL/6J |
Beijing HFK Bioscience Limited Company |
N/A |
|
| Genetic reagent (Mus musculus) |
Rag1-/-: B6.129S7-Rag1tm1/Nju |
Model Animal Research Center of Nanjing University |
Stock no :N000088 |
|
| Genetic reagent (Mus musculus) |
Tlr4-/- mice: C57BL/10ScNJNju |
Model Animal Research Center of Nanjing University |
Stock no: N000192 |
|
| Genetic reagent (Mus musculus) |
C57BL/10JNju |
Model Animal Research Center of Nanjing University |
Stock no: N000023 |
|
| Strain, strain background (Acinetobacter baumannii) |
LAC-4 |
Gift from Professor Chen, Harris et al., 2013
|
N/A |
|
| Strain, strain background (Acinetobacter baumannii) |
ATCC-17978 |
ATCC |
ATCC-17978 |
|
| Strain, strain background (Acinetobacter baumannii) |
SJZ-09 |
Clinical isolate, Zeng et al., 2019
|
|
|
| Strain, strain background (Acinetobacter baumannii) |
SJZ-24 |
Clinical isolate, Zeng et al., 2019
|
|
|
| Strain, strain background (Acinetobacter baumannii) |
SJZ-26 |
Clinical isolate, Zeng et al., 2019
|
|
|
| Strain, strain background (Pseudomonas aeruginosa) |
XN-1 |
Clinical isolate, Gao et al., 2020
|
|
|
| Strain, strain background (Klebsiella pneumoniae)
|
YBQ |
Clinical isolate, Liu et al., 2020
|
|
|
| Strain, strain background (Staphylococcus aureus)
|
KM-22 |
Clinical isolate, Zeng et al., 2020
|
|
|
| Strain, strain background (Streptococcus pneumoniae) |
S. pneumoniae
|
Clinical isolate |
|
|
| Antibody |
Rat monoclonal anti-mouse CD45-PE/Cy7 (clone 30-F11) |
BioLegend |
Cat# 103114 RRID:AB_312979
|
(1:100) |
| Antibody |
Rat monoclonal anti-mouse/human CD11b-PerCP/Cy5.5 (clone M1/70) |
BioLegend |
Cat# 101228 RRID:AB_893232
|
(1:100) |
| Antibody |
Rat monoclonal anti-mouse F4/80-APC (clone BM8) |
BioLegend |
Cat# 123116 RRID:AB_893481
|
(1:100) |
| Antibody |
Rat monoclonal anti-mouse Ly-6C-PE (clone HK1.4) |
BioLegend |
Cat# 128008 RRID:AB_1186132
|
(1:100) |
| Antibody |
Rat monoclonal anti-mouse Ly-6G-FITC (clone 1A8) |
BioLegend |
Cat# 127605 RRID:AB_1236488
|
(1:100) |
| Antibody |
Hamster monoclonal anti-mouse CD11c-PE (clone N418) |
BioLegend |
Cat# 117307 RRID:AB_313776
|
(1:100) |
| Antibody |
Rat monoclonal anti-mouse CD11c-FITC (clone N418) |
BioLegend |
Cat# 117305 RRID:AB_313774
|
(1:100) |
| Antibody |
Mouse monoclonal anti-mouse CD284(TLR4)-PE (clone UT41) |
Thermo Fisher |
Cat# 12-9041-80 RRID:AB_466236
|
(1:100) |
| Antibody |
Rat monoclonal rat IgG1 isotype control (clone HRPN) |
Bio X Cell |
Cat# BE0088, RRID:AB_1107775
|
200 μg/per mouse |
| Antibody |
Rat monoclonal anti-mouse TNF-α (clone XT3.11) |
Bio X Cell |
Cat# BE0058, RRID:AB_1107764
|
200 μg/per mouse |
| Sequence-based reagent |
Primers for qPCR |
This paper |
N/A |
See Supplementary file 1
|
| Commercial assay or kit |
RNAiso Plus |
Takara |
Cat# 9109 |
|
| Commercial assay or kit |
PrimeScript RT reagent kit |
Takara |
Cat# RR037A |
|
| Commercial assay or kit |
TB Green Premix Ex Taq II |
Takara |
Cat# RR820A |
|
| Commercial assay or kit |
IL-6 ELISA kit |
BioLegend |
Cat# 431304 |
|
| Commercial assay or kit |
TNF-α ELISA kit |
BioLegend |
Cat# 430904 |
|
| Commercial assay or kit |
IL-1β ELISA kit |
BioLegend |
Cat# 432604 |
|
| Commercial assay or kit |
Anti-mouse CD11c Microbeads kit |
Miltenyi Biotec |
Cat# 130-125-835 |
|
| Software, algorithm |
GraphPad Prism |
GraphPad Software |
RRID:SCR_002798
|
|
| software, algorithm |
FlowJo |
FlowJo |
RRID:SCR_008520
|
|
