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. 2021 Sep 10;10:e70372. doi: 10.7554/eLife.70372

Figure 3. WIPI2d Interfacial mutants decrease ATG16L1 binding.

Figure 3.

Key interacting residues shown as sticks in cartoon representation of WIPI2d:ATG16L1 interface shown from (A) the WIPI2d face or (B) down the ATG16L1 helix. (C) Inputs for the (D) Pull-down assays of mutant WIPI2d constructs and wild type with GST-ATG16L1 W2IR. GSH resin was used to pull-down GST-ATG16L1 W2IR from purified protein mixture. The pull-down results were performed in triplicates and visualized by SDS–PAGE and Coomassie blue staining.

Figure 3—source data 1. Uncropped SDS-PAGE gels for Figure 3.
Uncropped gel used in Figure 3C,D with lanes labeled similarly.