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. 2021 Sep 10;10:e70372. doi: 10.7554/eLife.70372

Figure 6. Reconstitution of membrane recruitment of WIPI2d mutants.

Figure 6.

(A) Representative confocal images of GUVs showing membrane binding of mCherry-WIPI2d. PI3KC3-C1, mCherry-WIPI2d WT or mutant, E3-GFP were incubated with GUVs (64.8% DOPC: 20% DOPE: 5% DOPS: 10% POPI: 0.2% Atto647 DOPE) at room temperature. Images taken at 30 min were shown. Scale bars, 10 µm. (B) Quantification of relative intensities of mCherry-WIPI2d on GUV membranes in (A) membranes (means ± SDs are shown; N = 40). (C) Quantification of confocal images of GUVs (69.8% DOPC: 20% DOPE: 5% DOPS: 5% DOPI(3)P: 0.2% Atto647 DOPE) showing membrane binding of mCherry-WIPI2d. mCherry-WIPI2d WT or mutant were incubated with GUVs at room temperature for 30 min and then imaged. (Means ± SDs are shown; N = 40). p≥0.5: (ns); 0.01<p<0.05: (*); 0.001<p<0.01: (**); p<0.001 (***); p<0.0001 (****).