| Strain, strain background (Saccharomyces cerevisiae) |
|
|
|
See Supplementary file 4
|
| Antibody |
Anti-FLAG M2 mouse monoclonal antibody |
Sigma |
RRID:AB_262044
|
(1:1000) |
| Antibody |
Goat anti-mouse IgG peroxidase |
Sigma |
RRID:AB_257993
|
(1:10,000) |
| Antibody |
Mouse anti-PGK1 monoclonal antibody |
Invitrogen |
RRID:AB_2532235
|
(1:1000) |
| Recombinant DNA reagent |
|
|
|
See Supplementary file 4
|
| Sequence-based reagent |
|
|
|
See Supplementary file 4
|
| Commercial assay or kit |
Dual-Luciferase Reporter Assay |
Promega |
Catalog# E1910 |
|
| Commercial assay or kit |
ERCC ExFold RNA Spike-In Mixes |
Invitrogen |
Catalog# 4456739 |
|
| Chemical compound, drug |
Cycloheximide |
Sigma |
Catalog# C1988 |
CAS# 66-81-9 |
| Chemical compound, drug |
Radicicol |
Research Products International |
Catalog#R20020-0.001 |
CAS# 12772-57-5 |
| Chemical compound, drug |
Rapamycin |
LC Laboratories |
Catalog# R-5000 |
CAS# 53123-88-9 |
| Chemical compound, drug |
Canavanine |
Sigma |
Catalog# C9758 |
CAS# 2219-31-0 |
| Chemical compound, drug |
5-Fluoroorotic acid monohydrate (5-FOA) |
GoldBio |
Catalog# F-230–2.5 |
CAS# 220141-70-8 |
| Chemical compound, drug |
Guanidine hydrochloride |
Sigma |
Catalog# G3272 |
CAS# 50-01-1 |
| Chemical compound, drug |
[35S]-methionine |
Perkin Elmer |
Catalog# NEG709A500UC |
|
| Chemical compound, drug |
N-Cyclohexyl-N′-(2-morpholinoethyl)carbodiimide methyl-p-toluenesulfonate (CMC) |
Sigma |
Catalog# C106402 |
CAS# 2491-17-0 |
| Software, algorithm |
ImageJ software |
ImageJ (http://imagej.nih.gov/ij/) |
RRID:SCR_003070
|
|
| Software, algorithm |
GraphPad Prism software |
GraphPad Prism (https://graphpad.com) |
RRID:SCR_002798
|
|
| Software, algorithm |
Model |
https://github.com/cjakobson/liveFastDieYoung
|
|
|
