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. Author manuscript; available in PMC: 2021 Sep 21.
Published in final edited form as: Methods Mol Biol. 2021;2293:163–179. doi: 10.1007/978-1-0716-1346-7_11

Figure 2. Conservation and function of the Rab40 SOCS box.

Figure 2.

A. Alignment of Rab40 SOCS box and other human SOCS proteins shows sequence conservation. Green highlights the Cul5 box, where the LPLP motif is conserved across all Rab40 sequences and multiple human SOCS box containing proteins. Conserved residues across all human SOCS box containing proteins are summarized at the bottom. B. Example of a Cullin-RING ligase (CRL) that includes the scaffold protein Cul5, a SOCS-containing adaptor protein, RING protein Rbx2, and adaptor proteins Elongin B and Elongin C (left). This CRL complex facilitates ubiquitylation of target substrates. Strong evidence from our lab and others shows that Rab40b (and other members of the Rab40 subfamily) is a legitimate SOCS-containing adaptor protein for Cul5 (right).