Figure 1.

Experimental procedure and data. (a,b) Explains the experimental procedure and boundary conditions for simulation models. (a) Photograph of six culture plates commonly used with a uniform monolayer of cells. (b) Schematic showing the uniform cell monolayer (shaded), scratched region (white) and imaged region (outlined in red) in a 35 mm culture plate. (c,d) Experimental images, both 1309.09 × 1745.35 μm, of WM983C FUCCI-transduced melanoma cells at 0 and 48 h, respectively. Images reproduced with permission from Vittadello et al. [20]. (e) Cell trajectory data of a select few cells recorded through red to green phases travelling inward to fill scratched region.