Figure 4.

Technical flowchart of scRNA-seq and snRNA-seq experiments on renal tissue of humans or mice, continued. ^IsnRNA-seq has not yet been performed on human core needle biopsies. ^IIMethanol fixation of single cells has not yet been performed on human renal tissue. ^IIIMagnetic bead isolation of glomeruli is not possible in human patients/tissue. ^IVManual selection of glomeruli before tissue dissociation has only been performed in one scRNA-seq study and still requires validation.^{18 V}FACS/MACS have successfully been used to enrich for glomerular endothelial cells in scRNA-seq experiments on mice,^44,50 but not yet for podocytes or mesangial cells. ^VICold dissociation protocols have been successfully used on renal tissue of mice,^33,34,45,52 but studies on human renal tissue have yet to be published. (+), advantage; (-), disadvantage.