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. 2021 Aug;32(8):1961–1973. doi: 10.1681/ASN.2020060777

Figure 1.

Figure 1.

Experimental design of the study. (A) High-density SNP genotyping determined that the B6.Cg-Col4a5tm1Yseg/J JAX stock #006183 strain contains 12 Mb of 129X1/SvJ genomic sequence surrounding the Col4a5 locus. (B) Generation of DO Col4a5 mutant models (DO-XLAS); 100 heterozygous female Col4a5 mice (B6.Cg-Col4a5tm1Yseg/J) were crossed with 100 male J:DO. From each mating, we used one F1 female that was heterozygous for the Col4a5 mutation and one F1 male that was hemizygous for the Col4a5 mutation for a total of 200 mice in the experimental cohort. (C) Experimental timeline with GFR measured at 14 weeks and ACR measured at 6, 10, and 15 weeks. Tissues were collected at 15 weeks of age.