Figure 1.

Consort diagram of the study and processing of small intestinal crypts/organoids. (A) Schematic representation of the study shows workflow of biopsy to crypt isolation, organoid culture, RNA extraction, and transcriptomic data analysis. (B) Immunofluorescence staining of small intestinal organoids for lysozyme (gold), E-cadherin (red), and DAPI (blue) showing epithelial-specific cell types. (C) Microscopic images of mature organoids in culture at 3 weeks are shown for both CD patients and non-IBD controls.