Figure 5.

Secretome analysis of PDO conditioned media. Thirty-plex secretome analysis was performed on 3-day-old organoid conditioned media. (A) Concentrations of 30 analytes from the supernatants of Control, Crohn’s disease (no inflammation; CD-NI), and Crohn’s disease inflammatory (CD-I) organoids are shown. Analyte concentrations in fresh organoid media (background reference) are also shown. Highlighted analytes are statistically significant over the organoid media (background reference). (B) Six of 30 analytes that are significantly higher in organoid condition media (O) over the background Organoid Medium (OM) are shown. Shown results are the cumulative of 2 independent Luminex 30-plex runs.