Fig. 4.

Motor coordination and muscle strength at different time points after single-dose administration of daridorexant in rats and comparison to zolpidem. Effect of a single dose of daridorexant (10, 30, 100 mg/kg), zolpidem (30 mg/kg), and vehicle (0.5% methylcellulose) on motor coordination (rotarod test) and muscle strength (grip strength test) in male Wistar rats. Following drug administration, rats were repeatedly tested at 30, 60, 90, 120, and 150 min post-dose. Baseline performance was measured 60 min before drug administration. Data are means ± standard error of the mean. N = 12 rats per dose group. **p < 0.01, ***p < 0.001 vs. vehicle (for each time point), with two-way repeated measure ANOVA followed by Dunnett’s multiple comparisons test. Data-on-file, Idorsia (see Online Resource S2 for experimental details)