FIGURE 10.

Effect of 2DG on the ability of HSV to reactivate from latently infected TG explanted cultures. BALB/c mice were infected ocularly with RFP/EGFP expressing HSV-1 KOS strain. TGs were removed from mice after the establishment of latency at 34 dpi. Explanted TGs were maintained either in complete DMEM media or media supplemented with 2DG (0.5 mM) for 8 days and the the supernatants were collected daily for viral titration and the media replaced. Panel A shows viral titer levels detected at different time points. Panel B shows viral expression differences in cultures with and without 2DG on day 4. Panel C shows viral reactivation differences on day 3 in TG cultures in normal media, media with 2DG as well as TG where both CD4 and CD8 T cells were depleted and 2DG added. A group of TGs were also cultured in glucose free media during reactivation (RFP/gC: red fluorescein protein under the control of late viral glycoprotein C of HSV-1; EGFP/ICP0: enhanced green fluorescein protein under the control of immediate early gene ICP0 of HSV-1; ns: not significant).