FIGURE 7.

Comparison of inflammatory cell responses in the TGs from 2DG treated and untreated HSV infected mice at day 9 pi. 8 TGs were harvested from each group and enzymatically dissociated to obtain single cell suspension. Single cells were harvested and surface stained with anti-CD45 fluorochrome-labeled antibodies. Histograms represent total CD45 expressing leucocyte cell numbers and frequencies in panel A and B, respectively. Macrophages (CD45⁺CD11b⁺F4/80⁺; plot C and D) and neutrophils (CD45⁺CD11b⁺Ly6G⁺; plots E and F) were also enumerated. FACS plots represent the frequencies of total leukocytes, macrophages and neutrophils from treated and untreated TGs after gating from live cell population. A two-tailed nonparametric Student t-test analysis was performed to estimate significant differences between 2DG treated and untreated groups (*p<0.05; ***p<0.001).