Figure 6.

NAC scavenges excessive ROS in LYCF cells exposed to hypoxia, alleviates oxidative toxicity, and promotes cell viability

A: Representative fluorescence photomicrographs of ROS (DCFH-DA, green), O₂^•− (DHE, red), and mitochondrial superoxide levels (MitoSOX RED, red) in LYCF cells with/without NAC pretreatment or hypoxia for 24 h. B, C: FACS analysis of ROS and O₂^•− levels in LYCF cells with/without NAC pretreatment or hypoxia for 24 h. D: H₂O₂ assay of changes in H₂O₂ levels in LYCF cells with/without NAC pretreatment or hypoxia for 24 h. E: Superoxide assay of changes in intracellular superoxide levels in LYCF cell with/without NAC pretreatment or hypoxia for 24 h. F–H: MDA, PCO, and 8-OHdG levels in LYCF cells with/without NAC pretreatment or hypoxia for 24 h. I: Viability of LYCF cells with/without 1 mmol/L/200 μmol/L/40 μmol/L NAC pretreatment or hypoxia for 24 h. ^*: P<0.05;^**: P<0.01.