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. 2021 Sep 16;2(4):100844. doi: 10.1016/j.xpro.2021.100844

Sucrose solution

Reagent Final concentration Amount
Nuclei PURE 2M Sucrose Cushion Solution N/A 2.7 mL
Nuclei PURE Sucrose Cushion Buffer N/A 0.3 mL

Prepare fresh and keep on ice.