| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Nuclei EZ lysis buffer | Sigma | Cat# NUC-101 |
| UltraPure™ BSA (50 mg/mL) | Thermo Fisher Scientific | Cat #AM2618 |
| SUPERase•In™ RNase Inhibitor (20 U/μL) | Thermo Fisher Scientific | Cat #AM2694 |
| Trypan Blue Solution, 0.4% | Thermo Fisher Scientific | Cat#15250061 |
| ProLong Diamond Antifade Reagent with DAPI | Life Technologies | Cat#P36971 |
| Neurobasal A Medium | Invitrogen | Cat#10888-022 |
| Ethidium Homodimer-1 | Invitrogen | Cat#E1169 |
| 1× PBS | Corning | Cat# 21040CM |
| Critical commercial assays | ||
| Nuclei PURE Prep Isolation Kit | Sigma | Cat# NUC201-1KT |
| RNA Screen Tape | Agilent Technologies | Cat# 5067-5576 |
| Chromium Single Cell 3′ kit | 10× Genomics | Cat#1000153 |
| Biological samples | ||
| Surgically resected human hippocampus tissue (mixed sex, 24–60 years old) | This study | N/A |
| Other | ||
| Eppendorf® Centrifuge 5810/5810R | Eppendorf | Cat# EP022628188 |
| 1.5 mL Eppendorf tubes | Eppendorf | Cat# 022431021 |
| 2 mL Eppendorf tubes | Eppendorf | Cat# EP022363344 |
| 15 mL conical tubes | Corning | Cat# CLS430791 |
| Dounce homogenizer | DWK Life Sciences | Cat# 8853000002 |
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