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. 2021 Feb 4;49(9):2103–2113. doi: 10.1007/s10439-021-02727-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of cell shape. Representative image of MDA-MB-231 cells stained with DAPI/Phalloidin after 7 days of culturing in static (a) or dynamic (b) conditions. (c) Eccentricity distribution of cell shape recovered segmenting the Phalloidin signal as detailed in the methods section (at least 10 images/condition). *p < 0.05.