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. 2021 Feb 4;49(9):2103–2113. doi: 10.1007/s10439-021-02727-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression analysis of MDA-MB-231 cells grown in 3D collagen scaffold (mRNA collected from 3 scaffolds per condition cultured in 2 independent experiments) for 4 days in static and dynamic conditions. The mRNA of invasion/migration related genes LOX, MMP2-3-9, RHOA, and VIM were analyzed by qPCR using ACTB and HPRT1 as reference genes (2^−ΔΔCT method). Fold changes respect to static condition were calculated.*p < 0.05; **p < 0.01.