Table 1.
Observed Box–Behnken Experimental Runs of DAC-Loaded BILS with Their Actual and Predicted Experimental Values of Y1, Y2, and Y3
| Runs | Independent Variables | Dependent Variables | PDI | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | Y1 (Mean ± SD) | Y2 (Mean ± SD) | Y3 (Mean ± SD) | |||||
| Actual | Predicted | Actual | Predicted | Actual | Predicted | |||||
| 1 | 3:1 | 15 | 30 | 312±16 | 304.8 | 87±3.39 | 87.5 | 43±1.24 | 43.2 | 0.30 |
| 2 | 3:1 | 0 | 10 | 395±19 | 402.5 | 85±2.65 | 85 | 47±2.02 | 45.7 | 0.30 |
| 3 | 7:1 | 30 | 10 | 480±15 | 472.5 | 95±1.79 | 95 | 33±3.61 | 32.7 | 0.20 |
| 4 | 5:1 | 0 | 5 | 408±11 | 402.6 | 90±1.73 | 90 | 39±1.15 | 41.7 | 0.20 |
| 5 | 5:1 | 15 | 10 | 310±11 | 316.6 | 97±1.40 | 96.3 | 39±2.35 | 39.2 | 0.30 |
| 6 | 5:1 | 30 | 30 | 290±10 | 295.0 | 95±1.78 | 95 | 35±1.50 | 36.7 | 0.20 |
| 7 | 7:1 | 0 | 10 | 470±17 | 468.0 | 89±1.93 | 89.5 | 40±2.14 | 40.2 | 0.50 |
| 8 | 5:1 | 30 | 5 | 390±14 | 390.0 | 97±1.94 | 97.5 | 35±2.74 | 34.2 | 0.20 |
| 9 | 3:1 | 30 | 10 | 370±26 | 371.7 | 89±1.92 | 88.5 | 37±4.01 | 38.2 | 0.30 |
| 10 | 5:1 | 15 | 10 | 320±26 | 316.6 | 97±1.37 | 96 | 41±3.05 | 39.2 | 0.30 |
| 11 | 7:1 | 15 | 30 | 385±14 | 387.0 | 92±1.41 | 92 | 38±2.00 | 37.7 | 0.30 |
| 12 | 7:1 | 15 | 5 | 475±13 | 482.0 | 93±1.21 | 92.5 | 35±2.25 | 35.2 | 0.27 |
| 13 | 3:1 | 15 | 5 | 400±17 | 397.8 | 86±2.47 | 86 | 41±2.83 | 40.7 | 0.29 |
| 14 | 5:1 | 0 | 30 | 310±8 | 309.6 | 94±2.24 | 93.5 | 44±4.00 | 44.2 | 0.29 |
| 15 | 5:1 | 15 | 10 | 320±18 | 316.6 | 95±2.89 | 96.3 | 41±4.11 | 39.2 | 0.30 |
Notes:A: ratio of PL:SDC; B: cholesterol amount (mg); C: sonication time (min); Y1: particle size (nm); Y2: EE (%); Y3: drug release (%).