Table 2.
Effect of metal ions or chemicals on the enzymatic activity of CapA.
| Metal ions or chemical reagents | Relative activity/% | ||
|---|---|---|---|
| 1 mmol L−1 | 5 mmol L−1 | 10 mmol L−1 | |
| Control | 100Ac ± 2.1 | 100Ac ± 1.3 | 100Ab ± 1.1 |
| Cu2+ | 20.7Af ± 0.3 | 9.3Bf ± 0.2 | 2.7Ce ± 0.1 |
| Mg2+ | 119.4Ba ± 3.6 | 127.5Aa ± 3.4 | 108.1Ca ± 2.6 |
| Fe2+ | 42.5Ae ± 1.6 | 16.4Be ± 1.4 | 7.5Cd ± 0.7 |
| Ca2+ | 99.4Ac ± 2.1 | 103.8Ac ± 3.7 | 98.7Ab ± 1.9 |
| Zn2+ | 97.9Ac ± 1.7 | 102.5Ac ± 3.3 | 96.3Ab ± 3.9 |
| Mn2+ | 96.5Ac ± 1.3 | 90.6Bd ± 2.8 | 52.1Cc ± 2.2 |
| Co2+ | 76.4Ad ± 1.3 | 10.4Bf ± 1.2 | 1.1Cf ± 0.1 |
| Na2+ | 106.4Ac ± 4.2 | 103.5Ac ± 5.2 | 104.8Ab ± 3.5 |
| EDTA | 107.4Ab ± 2.7 | 106.5Ab ± 1.8 | 107.7Aa ± 3.8 |
| PMSF | 14.9Ag ± 0.8 | / | / |
Values are mean ± standard deviation. Analysis performed in triplicate.
Different lowercase superscripts in the same column indicate statistically significant difference (p < 0.05) caused by different metal ions or chemicals within the same concentration. Capital letters in the same row indicate statistically significant difference (p < 0.05) caused by different concentration within the same metal ions or chemicals.
“/” means no detectable enzyme activity.