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. 2021 Sep 21;7:256. doi: 10.1038/s41420-021-00639-1

Fig. 1. Characterization and biodistribution of the multifunctional liposomes.

Fig. 1

a Schematic diagram of Tf-Lip and Tf-Pep63-Lip preparation. b–e Morphology and particle size of Tf-Lip and Tf-Pep63-Lip under a transmission electron microscope after negative staining with 2% uranyl acetate solution. c, e Magnifications of the framed areas of b and d. Scale bar: to the left panels, 50 nm; to the right panels, 10 nm. f, g HPLC analysis of the conjugation of Tf to liposomes at 280 nm shows a clear full shift from 6.4 min of non-conjugated Tf (f) to 3.6 min of conjugated Tf-Lip (g), indicating the successful conjugation of transferrin to liposomes. h–j Changes in the particle size of Tf-Lip and Tf-Pep63-Lip for seven days in NS (h), PBS (i), and ACSF (j) show stable physical properties in vitro (n = 3). k Biodistribution of Cy5.5-labeled and transferrin-modified liposomes in living mice. Cy5.5 signals, followed for up to 2 h of post-injection by live animal imaging after intravenous injection of 5 mg lipids/kg mouse of Cy5.5-Lip, Cy5.5-Tf-Lip, and the corresponding dose of Cy5.5 solution, show that transferrin-modified liposomes can cross the brain (n = 3). Data are presented as means ± SEM.