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. 2021 Sep 21;11:18750. doi: 10.1038/s41598-021-98407-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Muscle regeneration from the stumped muscle wall in granulation tissue. (A) Distribution of CD31, αSMA, myogenin, and myoglobin positive cells are shown in the granulation tissue with muscle regeneration. The seral sections were obtained from the Pro-Hyp group on day 14. Bars = 200 µm. (B) Electron micrographs of the abdominal lesion obtained from the Pro-Hyp group on day 14 showing myogenic differentiation in the granulation tissue. Myotubes forming a multinuclear envelope (left, arrows). Elongated myofibers with transverse striations (meddle, arrows). Large matured muscle fibers (right, dots). Bars = 20 µm.