Fig.3.

Fungal resistance assay with FoPEX6-RNAi and FoGAS1-RNAi transgenic tomato lines. Experiments were performed by inoculating the roots with fungal spores and disease severity index (DI) values were determined after 3 weeks for the transgenic lines relative to the wild-type control plants. DI values for wild-type control plants were recorded as 1.0, whereas transgenic lines have shown DI values ranging from 0.2 to 0.7; a lines 45 and 56 of FoPEX6RNAi with DI values ranging from 0.25 to 0.5 were 70–80% resistant. In contrast, line 46 was significantly less resistant with DI of 0.7 (30% resistant); b similarly, lines 7 and 15 of FoGAS1RNAi with DI values ranging from 0.3 to 0.5 were 70–80% resistant, while line 14 was 45–50% resistant with DI of 0.65; c Assessment of RNAi transgenic tomato lines for resistance against Fusarium wilt. Photographs were taken 3-week post-inoculation. Tomato seedlings (6 each from the wild-type and transgenic lines) were used for fungal resistance assays