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. 1999 Mar;37(3):792–795. doi: 10.1128/jcm.37.3.792-795.1999

TABLE 3.

Ultrasensitive quantification of samples not detecteda by the standard Amplicor HIV-1 Monitor test

Sample Ultrasensitive Amplicor HIV-1 Monitor test (HIV-1 RNA copies/ml) Standard Amplicor HIV-1 Monitor test
OD450b OD U above backgroundc
1 <50 0.171 0.101
2 <50 0.107 0.037
3 <50 0.278 0.208
4 <50 0.288 0.218
5 51 0.146 0.076
6 181 0.247 0.177
7 211 0.133 0.063
8 230 0.184 0.114
9 330 0.216 0.146
10 452 0.410 0.340
a

The detection limit of the Amplicor HIV-1 Monitor test is 400 copies/ml. 

b

Sample OD at 450 nm for a dilution factor of 1 in the assay detection step. 

c

Calculated by using sample OD − 0.070.